Analysis Note
ControlHuman embryonic stem cells
Application
Research CategoryStem Cell Research
Flow Cytometry:
Antibody dilution for cellular staining:
Prepare an antibody working solution by diluting 1:5 the primary antibody with PBS.
Dispense the volume per test of working of solution according to the number of cells indicated in the table below.
5 µL for Guava Flow Cytometer
10 µL for other Flow Cytometry instruments
| Working Solution | Cells / test | Total Reaction Volume |
|---|
| 5 µl | 5 x 105 Cells / 95 µl PBS | 100 µl |
| 10 µl | 1 x 106 Cells / 90 µl PBS | 100 µl |
Detect Oct-4 using this Anti-Oct-4 Antibody, clone 10H11.2, Alexa Fluor 488 conjugate validated for use in FC & IC.
Research Sub CategoryPluripotent & Early Differentiation
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
General description
Octamer-4 (Oct-4), a member of the POU family of transcription factors, has been demonstrated to be vital for the formation of self-renewing pluripotent stem cells. During embryogenesis expression of Oct-4 is limited to pluripotent cells of the inner cell mass (ICM) that contribute to the formation of all fetal cell types. This relationship between Oct-4 and pluripotency has seen this transcription factor emerge as a marker of pluripotent stem cells. Undifferentiated human and murine pluripotent embryonic stem (ES) and embryonic carcinoma (EC) cells express Oct-4. Additionally, murine embryonic germ (EG) cells are also known to express Oct-4. Following stem cell differentiation, the level of Oct-4 expression decreases. Oct-4 expression by human EG cells is not known.
Immunogen
Recombinant GST fusion protein, human Oct-4 (aa 1-134).
Legal Information
ALEXA FLUOR is a registered trademark of Life Technologies
Physical form
Purified mouse monoclonal IgG1 conjugated to Alexa Flour™ 488 in PBS with less than 0.09% sodium azide and 15 mg/mL BSA.
Quality
Evaluated by Flow Cytometry in 2102Ep Cells.
Specificity
Reacts with human Oct-4.
Storage and Stability
Maintain refrigerated at 2-8°C protected from light in undiluted aliquots for up to 6 months from date of receipt
Target description
~44 kDa
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